Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor.
نویسندگان
چکیده
A general approach to mimic the sensing scheme of allosteric enzymes is developed. Through the covalent labeling of a sulfonamide inhibitor to the enzyme HCAII via SNAP-tag protein, the enzyme is rendered inactive. Catalytic activation is triggered only when a protease is present to cleave the recognized peptide sequence.
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عنوان ژورنال:
- Chemical communications
دوره 49 55 شماره
صفحات -
تاریخ انتشار 2013